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Hiroaki Fujii (藤井 宏昭, Fujii Hiroaki) is a former Japanese diplomat and current President of the Japan Foundation. He studied at the University of Tokyo , Amherst College and Harvard University .
Docent, Department of Life Technologies, Senior Research Fellow, Molecular Plant Biology Ph.D hiroaki.fujii@utu.fi +358 50 574 3891.
Nov 15, 2021 · Hiroaki Fujii. University of Arizona: Tucson, AZ, US. 2003-05 to 2003-12. Employment. Show more detail.
Hiroaki FUJII | Cited by 10,698 | of University of Turku, Turku (UTU) | Read 37 publications | Contact Hiroaki FUJII
- Abstract
- Introduction
- Results
- Discussion
- Methods
- Acknowledgments
The phytohormone abscisic acid (ABA) regulates various plant processes, including seed germination and seedling growth. Phosphorylation has been suggested to be important in ABA signaling, but little is known about the protein kinases needed. This study shows that two protein kinases, SnRK2.2 and SnRK2.3, act redundantly to control responses to ABA...
The phytohormone abscisic acid (ABA) is a key factor in regulating developmental and physiological processes in plants, including seed dormancy and germination and seedling growth, as well as in controlling many abiotic stress responses (Leon-Kloosterziel et al., 1996; Schroeder et al., 2001; Bray, 2002; Finkelstein and Gibson, 2002; Zhu, 2002; Ass...
snrk2.2 snrk2.3 Is Insensitive to ABA in Seed Germination
When we analyzed ABA responses in snrk2.2, snrk2.3, and snrk2.2 snrk2.3, snrk2.2 snrk2.3 was found to be ABA-insensitive in seed germination and early seedling growth. This was true in assays measuring the emergence of green cotyledons (Figures 1C to 1E) as well as when germination was scored by radicle emergence (Figure 1F). In all of these assays, the snrk2.2 and snrk2.3single mutants showed either no significant difference from the wild type or a much weaker ABA-insensitive phenotype relat...
snrk2.2 snrk2.3 Is Also Insensitive to ABA Regulation in Seedling Growth
As was the case for seed germination, no difference between snrk2.6 and the wild type was observed in the sensitivity of seedling growth to ABA (see Supplemental Figure 1 online).
SnRK2.2 and SnRK2.3 Are Expressed in Various Tissues
SnRK2.6, which plays a role in stomatal regulation, is expressed specifically in guard cells (Mustilli et al., 2002; Yoshida et al., 2002). Because snrk2.2 snrk2.3 showed impairment in various aspects of ABA responses, the expression patterns of SnRK2.2 and SnRK2.3were analyzed.
ABA regulates many important plant processes, including seed germination, dormancy, seedling growth, and stomatal aperture. Although many genes have been reported to be involved in ABA signaling, some key components are still missing, and ABA signaling appears to involve a highly branched network. SnRK2.6 has been identified as a positive regulator...
T-DNA Insertion Lines
The seeds of Arabidopsis thaliana snrk2.2 and snrk2.3 T-DNA insertion lines (GABI-Kat 807G04 and Salk_107315) were obtained from the Max Planck Institute for Plant Breeding Research (Rosso et al., 2003) and the ABRC (Alonso et al., 2003), respectively. Homozygous plants were obtained by PCR screening using primers designed by the I-Sect website (http://signal.salk.edu/cgi-bin/tdnaexpress; primer sequences are given Supplemental Table 1 online). The snrk2.6 mutant used here is the original ost...
Production of Transgenic Plants and GUS Staining
Fragments covering 2 kb upstream of the translational start sites of SnRK2.2, SnRK2.3, and SnRK2.6 were amplified by PCR using genomic DNA as templates. For SnRK2.2 and SnRK2.3, this region contains endogenous HindIII or BamHI sites. Therefore, two different fragments covering the 2-kb region were amplified, with each fragment having different portions of HindIII and BamHI sites on each end such that mixing, denaturing, and reannealing the fragments generated heterodimers with HindIII/BamHI c...
Kinase Assays
The ABF2, ABI5, and ABF1 fragments used for in vitro kinase assays were obtained by reverse transcription and amplification of the portion of the ABF2, ABI5, or ABF1 cDNA corresponding to amino acids Gly-73 to Gln-119 of ABF2, Arg-132 to Gln-190 of ABI5, and Gly-83 to Glu-131 of ABF1. The template for cloning was total RNA extracted from 12-d-old seedlings or genomic DNA of Col-0. The PCR product was digested with BamHI and XhoI (ABF2 and ABF1) or EcoRI (ABI5) and subcloned into pGEX4T1 vecto...
We thank Rebecca Stevenson for technical assistance. We also thank the ABRC and the Max Plank Institute for Plant Breeding Research for providing the T-DNA insertion mutants. This work was supported by National Institutes of Health Grant R01 GM-059138 and National Science Foundation Grant IBN-0420152 to J.-K.Z. P.E.V. was supported by National Inst...
- Hiroaki Fujii, Paul E. Verslues, Jian-Kang Zhu
- 2007
Hiroaki Fujii's 33 research works with 2,004 citations and 1,926 reads, including: A case of Krukenberg carcinoma metastasized from colon cancer resembling mucinous cystadenocarcinoma...
Hiroaki Fujii. Biology, Environmental Science. Methods in molecular biology. 2024; TLDR. The purification of recombinant proteins produced in Escherichia coli ...
