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  1. Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Protein Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • Nucleotide Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

  2. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query.

  3. BLAST is one of the most widely used bioinformatics programs for sequence searching. [4] It addresses a fundamental problem in bioinformatics research. The heuristic algorithm it uses is much faster than other approaches, such as calculating an optimal alignment.

  4. ElasticBLAST is a suite of command line applications that distributes your BLAST searches across multiple instances in the cloud. It can run large numbers of queries faster than BLAST+ on a single machine and manage cloud resources for you.

  5. BLAST is a computer algorithm that can rapidly align and compare a query DNA sequence with a database of sequences. Learn how BLAST works, its applications in genomic research, and its history and development.

  6. Mar 17, 2014 · Learn how to use blastn, a tool for comparing nucleotide sequences (DNA and RNA), with this interactive text and activity. Find out how to interpret the E value and alignment scores, and explore the kingdom of life with unknown sequences.

  7. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses).

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