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The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. BlastP simply compares a protein query to a protein database. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run.
Magic-BLAST ¶ Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome. Read more about Magic-BLAST at NCBI Magic-BLAST Documentation. Installers and source code are available from https://ftp.ncbi.nlm.nih.gov/blast/executables/magicblast/LATEST. IgBLAST ¶
Primer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets.
Jul 1, 2004 · Basic Local Alignment Search Tool (BLAST) is a sequence similarity search program that can be used via a web interface or as a stand-alone tool ( 1, 2 ). There are several types of BLAST to compare all combinations of nucleotide or protein queries with nucleotide or protein databases.
The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses).
BLAST (basic local alignment search tool) is a tool for comparing primary biological sequence information such as the amino-acid sequences of proteins. A BLAST search enables a researcher to compare a subject protein (called a query) with a database of sequences, and identify database sequences that resemble the query sequence above a certain ...
Mar 21, 2024 · The Basic Local Alignment Search Tool (BLAST) finds regions of similarity between sequences. The program compares nucleotide or protein sequences and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
Awash in a sea of data, how do scientists identify the function of a newly cloned gene? Online resources like the Basic Local Alignment Search Tool (BLAST) provide a helping hand.
Aug 3, 2023 · BLAST stands for Basic Local Alignment Search Tool. It is a widely used bioinformatics program that was first introduced by Stephen Altschul et al. in 1990 and has since become one of the most popular tools for sequence similarity search.
