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  1. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query. DELTA-BLAST constructs a PSSM using the results of a Conserved Domain Database search and searches a sequence database.

  2. Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify ...

  3. BLAST. ®. Command Line Applications User Manual. Bethesda (MD): National Center for Biotechnology Information (US); 2008 -. This manual documents the BLAST (Basic Local Alignment Search Tool) command line applications developed at the National Center for Biotechnology Information (NCBI).

  4. BLAST is an acronym for Basic Local Alignment Search Tool and refers to a suite of programs used to generate alignments between a nucleotide or protein sequence, referred to as a “query” and nucleotide or protein sequences within a database, referred to as “subject” sequences. The original BLAST program used a protein “query ...

  5. NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. BLAST® Help [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2008-.

  6. Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type. Help. Entrez query (optional) Help. Primer specificity stringency. Primer must have at least total mismatches to unintended targets, including.

  7. Jun 23, 2008 · BLAST® Command Line Applications User Manual [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2008-. BLAST ® Command Line Applications User Manual [Internet].