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Our UV dicing tapes feature a polyethylene or polyvinyl chloride base film and UV releasable adhesive to secure silicon wafers during dicing and die sorting processes. They provide excellent cutting performance and particle-free dicing with stable wafer fixing.
The Direct Credit Authorisation (DCA) Form is for an organisation/individual to request for payments from the Government of Singapore by direct credit into a designated bank account. The same form can be used for new creation or amendment. You will need the following information to complete the form:
- 35KB
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- Cell Culture
- Compounds
- Cell Viability and Cell Proliferation Assays
- Confirmation of Synergism
- Membrane Lipid Oxidation Rate
- Western Blot Analysis
- Metabolic Assays
- Statistical Analysis
The breast cancer cell lines MCF-7 and MDA MB-231 were a kind gift from Göran Landberg (Sahlgrenska Cancer Center, University of Gothenburg, Gothenburg, Sweden). The colon cancer cell line HT-29, the hepatocellular cancer cell line HEPG2, the cervical cancer cell line HeLa and the adenocarcinoma lung cancer cell lines A549 and H441, as well as the ...
PX-478 (Hölzel Diagnostika Handels GmbH, Cologne, Germany) and DCA (Sigma-Aldrich, Munich, Germany) were dissolved in distilled water.
A total of 0.75 × 104 A549, 1 × 104 HEK-293, 0.3 × 104 HeLa, 0.6 × 104 HEPG2, 1.5 × 104 HT-29, 0.5 × 104 MCF-7, 1.5 × 104 MDA-MB-231, 1 × 104 H441 and 0.3 × 104 U251 cells per well were seeded in flat bottom 96-well plates. After 24 h, when the cells were approximately 50% confluent, DCA, PX-478 or the combination was added. After 48 h of further i...
Synergism was evaluated with four to seven different concentrations (mostly with 6), as suggested by Chou and Talalay . Cells were treated with the combination of DCA and PX-478 at a constant EC50:EC50 ratio as well as with the single compounds alone. Significant differences between each single compound and the combination were assessed by an unpai...
HT-29 cells were seeded in 10 cm diameter Petri dishes and treated with the EC50 dose of DCA, the EC50 dose of PX-478 or the combination after 24 h when the cells were approximately 80% confluent. After incubation for an additional 48 h, cells were harvested with trypsin, pelleted and resuspended in 500 μl of PBS. For lipid extraction, cells were h...
For Western blotting, cells were seeded in 6 cm diameter Petri dishes, grown to approximately 80% confluence and treated with the noted compounds. 24 h later, the cells were washed with PBS and lysed with lysis buffer (50 mM β-glycerophosphate pH 7.6, 1.5 mM EGTA, 1.0 mM EDTA, 1% (v/v) Triton X-100, 0.2% (v/v) protease inhibitor cocktail, 0.4% (v/v...
MCF-7 cells were seeded in an XF 96-well culture microplate (Agilent, Santa Clara, USA) at 3 × 104 cells per well in 180 μl of prewarmed assay medium. After 24 h, a mitochondrial respiration assay or glycolytic rate assay was performed with a Seahorse XFe96 Analyzer (Agilent Technologies). For the mitochondrial respiration assay, the oxygen consump...
Statistical analysis was performed using unpaired T-tests in GraphPad Prism 7.05 statistical analysis software. Differences with a p-value of ≤0.05 were considered significant: significant differences compared to the control are marked with an asterisk (*), while significant differences between the combination and both the control and each single c...
- Jonas Parczyk, Jérôme Ruhnau, Carsten Pelz, Max Schilling, Hao Wu, Nicole Nadine Piaskowski, Britta ...
- 2021
Mar 25, 2015 · A hybrid drug dichloroacetate-platinum (II) [DCA-Pt (II)] was found to overcome cisplatin drug resistance of ovarian cancer through a dual targeting mode, which is different from the...
Dec 28, 2021 · US-responsive DDSs require biocompatible materials as carriers capable of releasing or activating therapeutic drugs through specific protonation, hydrolysis, phase transition, and molecular or supramolecular conformational changes.
Jan 6, 2022 · DCA targets cancer cells and inhibits pyruvate dehydrogenase kinase, the inhibitor of pyruvate dehydrogenase. Therefore, DCA alters the metabolism of tumors from glycolysis towards oxidative phosphorylation . Activation of PDH induces pyruvate mitochondrial oxidation and limits the metabolic advantage of tumor cells.
Nov 14, 2019 · An extensive body of literature describes anticancer property of dichloroacetate (DCA), but its effective clinical administration in cancer therapy is still limited to clinical trials. The occurrence of side effects such as neurotoxicity as well as the suspicion of DCA carcinogenicity still restricts the clinical use of DCA.
