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May 30, 2024 · Learn about the microscope, an instrument that produces enlarged images of small objects, from optical, electron, acoustic, X-ray, and other types. Explore the history, parts, diagram, and facts of microscopy with Britannica.
Oct 19, 2023 · Learn about the history, types, and uses of microscopes, instruments that magnify small objects and cells. Find out how lenses, magnification, and light work together to create detailed images of the microscopic world.
Review the principles of light microscopy and identify the major parts of the microscope. Learn how to use the microscope to view slides of several different cell types, including the use of the oil immersion lens to view bacterial cells.
- Overview
- Introduction
- Microscopes and lenses
- Light microscopes
- Electron microscopes
Introduction to microscopes and how they work. Covers brightfield microscopy, fluorescence microscopy, and electron microscopy.
If you meet some cell biologists and get them talking about what they enjoy most in their work, you may find it comes down to one thing: secretly, they’re all microscope freaks. At the end of the day, what they really love is the chance to sit in a small, dark room for hours on end, communing with their favorite cell type through the lens of a beautiful microscope. That may seem odd, but the truth is, cells can be pretty gorgeous, like living stained glass. One of my favorite examples of this is the picture below, which shows cells in a very young leaf of thale cress, a small flowering plant related to mustard.
This picture isn’t a plain light micrograph; it’s a fluorescent image of a specially prepared plant where various parts of the cell were labeled with tags to make them glow. However, this kind of cellular complexity and beauty is all around us, whether we can see it or not.
Although cells vary in size, they’re generally quite small. For instance, the diameter of a typical human red blood cell is about eight micrometers (0.008 millimeters). To give you some context, the head of a pin is about one millimeter in diameter, so about 125 red blood cells could be lined up in a row across the head of a pin. With a few exceptions, individual cells cannot be seen with the naked eye, so scientists must instead use microscopes (micro- = “small”; -scope = “to look at”) to study them. A microscope is an instrument that magnifies objects otherwise too small to be seen, producing an image in which the object appears larger. Most photographs of cells are taken using a microscope, and these pictures can also be called micrographs.
From the definition above, it might sound like a microscope is just a kind of magnifying glass. In fact, magnifying glasses do qualify as microscopes; since they have just one lens, they are called simple microscopes. The fancier instruments that we typically think of as microscopes are compound microscopes, meaning that they have multiple lenses. Because of the way these lenses are arranged, they can bend light to produce a much more magnified image than that of a magnifying glass.
In a compound microscope with two lenses, the arrangement of the lenses has an interesting consequence: the orientation of the image you see is flipped in relation to the actual object you’re examining. For example, if you were looking at a piece of newsprint with the letter “e” on it, the image you saw through the microscope would be “ə." 1 More complex compound microscopes may not produce an inverted image because they include an additional lens that “re-inverts” the image back to its normal state.
What separates a basic microscope from a powerful machine used in a research lab? Two parameters are especially important in microscopy: magnification and resolution.
•Magnification is a measure of how much larger a microscope (or set of lenses within a microscope) causes an object to appear. For instance, the light microscopes typically used in high schools and colleges magnify up to about 400 times actual size. So, something that was 1 mm wide in real life would be 400 mm wide in the microscope image.
•The resolution of a microscope or lens is the smallest distance by which two points can be separated and still be distinguished as separate objects. The smaller this value, the higher the resolving power of the microscope and the better the clarity and detail of the image. If two bacterial cells were very close together on a slide, they might look like a single, blurry dot on a microscope with low resolving power, but could be told apart as separate on a microscope with high resolving power.
Most student microscopes are classified as light microscopes. In a light microscope, visible light passes through the specimen (the biological sample you are looking at) and is bent through the lens system, allowing the user to see a magnified image. A benefit of light microscopy is that it can often be performed on living cells, so it’s possible to watch cells carrying out their normal behaviors (e.g., migrating or dividing) under the microscope.
Student lab microscopes tend to be brightfield microscopes, meaning that visible light is passed through the sample and used to form an image directly, without any modifications. Slightly more sophisticated forms of light microscopy use optical tricks to enhance contrast, making details of cells and tissues easier to see.
Another type of light microscopy is fluorescence microscopy, which is used to image samples that fluoresce (absorb one wavelength of light and emit another). Light of one wavelength is used to excite the fluorescent molecules, and the light of a different wavelength that they emit is collected and used to form a picture. In most cases, the part of a cell or tissue that we want to look at isn't naturally fluorescent, and instead must be labeled with a fluorescent dye or tag before it goes on the microscope.
The leaf picture at the start of the article was taken using a specialized kind of fluorescence microscopy called confocal microscopy. A confocal microscope uses a laser to excite a thin layer of the sample and collects only the emitted light coming from the target layer, producing a sharp image without interference from fluorescent molecules in the surrounding layers4 .
Some cutting-edge types of light microscopy (beyond the techniques we discussed above) can produce very high-resolution images. However, if you want to see something very tiny at very high resolution, you may want to use a different, tried-and-true technique: electron microscopy.
Electron microscopes differ from light microscopes in that they produce an image of a specimen by using a beam of electrons rather than a beam of light. Electrons have much a shorter wavelength than visible light, and this allows electron microscopes to produce higher-resolution images than standard light microscopes. Electron microscopes can be used to examine not just whole cells, but also the subcellular structures and compartments within them.
One limitation, however, is that electron microscopy samples must be placed under vacuum in electron microscopy (and typically are prepared via an extensive fixation process). This means that live cells cannot be imaged.
In the image above, you can compare how Salmonella bacteria look in a light micrograph (left) versus an image taken with an electron microscope (right). The bacteria show up as tiny purple dots in the light microscope image, whereas in the electron micrograph, you can clearly see their shape and surface texture, as well as details of the human cells they’re trying to invade.
There are two major types of electron microscopy. In scanning electron microscopy (SEM), a beam of electrons moves back and forth across the surface of a cell or tissue, creating a detailed image of the 3D surface. This type of microscopy was used to take the image of the Salmonella bacteria shown at right, above.
In transmission electron microscopy (TEM), in contrast, the sample is cut into extremely thin slices (for instance, using a diamond cutting edge) before imaging, and the electron beam passes through the slice rather than skimming over its surface5 . TEM is often used to obtain detailed images of the internal structures of cells.
Microscopy has applications in the forensic sciences. The microscope can detect, resolve and image the smallest items of evidence, often without any alteration or destruction. The microscope is used to identify and compare fibers, hairs, soils, and dust...etc.
May 26, 2024 · What are Microscopes? Microscopes are instruments that are used in science laboratories to visualize very minute objects, such as cells and microorganisms, giving a contrasting image that is magnified. Microscopes are made up of lenses for magnification, each with its own magnification powers.
Aug 19, 2019 · Learn how the microscope revolutionised medicine and biology, from the first compound microscopes to the electron microscope. Explore the stories of pioneers like Leeuwenhoek, Hooke, Virchow and Hassall, and their discoveries of cells, tissues and diseases.
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