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5 days ago · The two endogenous miRNA controls were identified from the nCounter assay data by Cook et al. 30 using NormFinder 45 which incorporates the inter- and intragroup variances and calculates the stability value per miRNA target as a measure of expression stability. It also identifies the best combination of miRNA targets to provide the highest ...
1 day ago · Figure 2. First, miRNA genes are transcribed into primary miRNA (pri-miRNA) within the nucleus. Then, under the action of nuclease Drosha and its cofactor Pasha, pri-miRNA is further processed to form a precursor miRNA (pre-miRNA), which is then transported into the cytoplasm via the GTP-dependent Exprotin-5 complex.
2 days ago · How to analyze miRNA-seq datasets alone or both miRNA and corresponding mRNA datasets together; How to use QIAGEN IPA without a dataset, using miRNA IDs; Introduction to databases and curated content specific to miRNA; How to effectively apply various filters and functionalities to identify biomarkers, key targets and biological mechanisms
1 day ago · In the miRNA pathway's cytoplasmic part, the miRNA is processed from a hairpin-structured precursor to a double-stranded (ds) mature RNA and ultimately to a single-stranded mature miRNA. In insects, ingesting these two ds forms can regulate the target gene expression; this inspired the trophic miRNA's use as a functional genomics and pest management tool.
2 days ago · The presence of residual microRNAs in purified rAAV batches was investigated. MiRNAs contaminate AAV vectors regardless of production cell platform or serotype. Immunoaffinity purification and RNase treatment are effective in reducing their abundancy. Further research is necessary to assess the impact of miRNA contaminants on AAV vectors safety and efficacy.
2 days ago · miRNA Family and Sample Correlation Analysis. Family analysis was conducted on the detected known miRNAs and novel predicted miRNAs, and the results showed that the known miRNAs and novel predicted miRNAs belong to a total of 31 different miRNA families, with miR-10 having the most abundant distribution (Fig. 2).
3 days ago · Abstract. Methods for sequence-specific microRNA (miRNA) analysis are crucial for miRNA research and guiding nursing strategies. We have devised a colorimetric technique for detecting miRNA using a dumbbell probe-based polymerase/endonuclease assisted chain displacement, along with silver ions (Ag +) aptamer assisted color reaction.
