Search results
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe. PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body.
- PBS Chemical Composition
- Protocol For Making Phosphate-Buffered Saline
- Sterilization and Storage of PBS Solution
- Diluting A 10X Solution to Make 1X PBS
- PBS Versus DPBS
- Sources
There are several recipes to prepare PBS solution. The essential solution contains water, sodium hydrogen phosphate, and sodium chloride. Some preparations contain potassium chloride and potassium dihydrogen phosphate. EDTA may also be added in cellular preparation to prevent clumping. Phosphate-buffered saline is not ideal for use in solutions tha...
Depending on your purpose, you may prepare 1X, 5X, or 10X PBS. Many people simply purchase PBS buffer tablets, dissolve them in distilled water, and adjust the pH as needed with hydrochloric acid or sodium hydroxide. However, it's easy to make the solution from scratch. Here are recipes for 1X and 10X phosphate-buffered saline: 1. Dissolve the reag...
Sterilization isn't necessary for some applications, but if your are sterilizing it, dispense the solution into aliquots and autoclave for 20 minutes at 15 psi (1.05 kg/cm2) or use filter sterilization. Phosphate-buffered saline may be stored at room temperature. It may also be refrigerated, but 5X and 10X solution may precipitate when cooled. If y...
10X is a concentrated or stock solution, which may be diluted to make a 1X or normal solution. A 5X solution must be diluted 5 times to make a normal dilution, while a 10X solution must be diluted 10 times. To prepare a 1 liter working solution of 1X PBS from a 10X PBS solution, add 100 ml of the 10X solution to 900 ml of water. This only changes t...
Another popular buffer solution is Dulbecco's phosphate buffered saline or DPBS. DPBS, like PBS, is used for biological research and buffers in the 7.2 to 7.6 pH range. It can be stored at room temperature. Dulbecco's solution contains a lower concentration of phosphate. It is 8.1 mM mM phosphate ions, while regular PBS is 10 mM phosphate. The reci...
Dulbecco, R.; et al. (1954). "Plaque formation and isolation of pure lines with poliomyelitis viruses". J. Exp. Med.99 (2): 167–182."Phosphate-buffered saline (PBS." Cold Spring Harbor Protocols (2006). Cold Spring Harbor Laboratory Press.- Anne Marie Helmenstine, Ph.D.
Jun 2, 2019 · Learn how to prepare PBS, a water-based salt solution used for biological research and immunohistochemical staining. Follow the recipe, tips and uses of PBS buffer.
Phosphate-buffered saline (PBS) PBS can be made as a 1× solution or as a 10× stock. To prepare 1 L of either 1× or 10× PBS, dissolve the reagents listed above in 800 mL of H 2 O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H 2 O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at ...
Sep 28, 2022 · PBS solution is a water-based salt solution used for washing, diluting, and transporting cells and tissues in biological research. Learn what PBS stands for, how to prepare it, and what are its advantages and limitations.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS supports cell viability by keeping the medium pH and osmolality near-physiological. Several formulations of PBS have been described.
